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At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA is 0.020 (μg/mL) −1 cm −1, for single-stranded DNA it is 0.027 (μg/mL) −1 cm −1, for single-stranded RNA it is 0.025 (μg/mL) −1 cm −1 and for short single-stranded oligonucleotides it is dependent on the length and base composition.
In biochemistry, the molar absorption coefficient of a protein at 280 nm depends almost exclusively on the number of aromatic residues, particularly tryptophan, and can be predicted from the sequence of amino acids. [6] Similarly, the molar absorption coefficient of nucleic acids at 260 nm can be predicted given the nucleotide sequence.
Once a reference signal is acquired, it is compared to the transmitted light Is of the measured sample. In order to calculate the associated extinction coefficient μ t, Beer's law can be applied as follows: I s = I o exp(-μ t d), where d is the sample thickness. The extinction coefficient is therefore: μ t = -ln(I s /I o)/d.
The following table is a representative sample of Erwin Chargaff's 1952 data, listing the base composition of DNA from various organisms and support both of Chargaff's rules. [17] An organism such as φX174 with significant variation from A/T and G/C equal to one, is indicative of single stranded DNA.
Sodium dodecyl sulfate (SDS), a common detergent, may be found in protein extracts because it is used to lyse cells by disrupting the membrane lipid bilayer and to denature proteins for SDS-PAGE. While other detergents interfere with the assay at high concentration, the interference caused by SDS is of two different modes, and each occurs at a ...
Esculentin-2CHa (from Latin ēsculentus (“edible; nutritious; full of food”)) is an antimicrobial peptide located outside the epithelial cell's membrane of the skin of many species of amphibians, such as Rana chiricahuensis. This peptide has recently become more important due to its defense response function and its possible application in ...
"He wouldn't let me go inside. And he choked me (unintelligible) in the hallway," she said. "He blocked the door so I couldn't go inside, and when I did go inside, he chased me upstairs.
The mass attenuation coefficient (also called "mass extinction coefficient"), which is the absorption coefficient divided by density; The absorption cross section and scattering cross-section, related closely to the absorption and attenuation coefficients, respectively "Extinction" in astronomy, which is equivalent to the attenuation coefficient