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For these purposes, hepatocytes are usually isolated from animal or human [8] whole liver or liver tissue by collagenase digestion, which is a two-step process. In the first step, the liver is placed in an isotonic solution, in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent.
54426 Ensembl ENSG00000109758 ENSMUSG00000029102 UniProt Q04756 Q9R098 RefSeq (mRNA) NM_001297439 NM_001528 NM_019447 RefSeq (protein) NP_001284368 NP_001519 NP_062320 Location (UCSC) Chr 4: 3.44 – 3.45 Mb Chr 5: 35.2 – 35.21 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Hepatocyte growth factor activator is a protein that in humans is encoded by the HGFAC gene. The protein ...
HepaRG cell line is a human hepatic in vitro line used in liver biology research and for assessing liver pathology, hepatotoxicity, and drug-induced injury. The HepaRG model is considered a surrogate for Primary Human Hepatocytes, which are the most pertinent model to reproduce the human liver functioning as they express 99% of the same genes .
In histology (microscopic anatomy), the lobules of liver, or hepatic lobules, are small divisions of the liver defined at the microscopic scale. The hepatic lobule is a building block of the liver tissue, consisting of portal triads, hepatocytes arranged in linear cords between a capillary network, and a central vein.
Apoptosis of the liver of a mouse. Hepatocytes are the liver's primary parenchymal cells, forming 80% of the liver's mass and 60% of its cells. They are round in shape and contain a nucleus and organelles that contribute to metabolic and secretory functions. [5] Hepatocytes also play a pivotal role in liver inflammation. [6]
Hepatic stellate cells (HSC), also known as perisinusoidal cells or Ito cells (earlier lipocytes or fat-storing cells), are pericytes found in the perisinusoidal space of the liver, also known as the space of Disse (a small area between the sinusoids and hepatocytes). The stellate cell is the major cell type involved in liver fibrosis, which is ...
Histopathology of a ballooning hepatocyte.png, H&E stain. Ballooning degeneration centre-left and centre-right. H&E stain. A Councilman body can also be seen in the upper-right of the section. In histo pathology, ballooning degeneration, formally ballooning degeneration of hepatocytes, is a form of liver parenchymal cell (i.e. hepatocyte) death.
In line dancing the term chassé is used for a triple-step sequence in any direction (forward, side, back, diagonally, or curving). For instance, if the chassé is to be done to the right, the right foot steps right, the left foot is placed next to the right, with the weight being transferred to the left foot so that the right foot can complete ...