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A clear CLED agar plate after cultivation Colonies of lactose and non-lactose fermenting bacteria on CLED agar. CLED agar (cystine–lactose–electrolyte-deficient agar or medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary microbes.
Diagnostic microbiology is the study of microbial identification. Since the discovery of the germ theory of disease , scientists have been finding ways to harvest specific organisms. Using methods such as differential media or genome sequencing , physicians and scientists can observe novel functions in organisms for more effective and accurate ...
Buffered charcoal yeast extract (BCYE) agar is a selective growth medium used to culture or grow certain types of bacteria, particularly the Gram-negative species Legionella pneumophila. [1]
Lysine iron agar or LIA is a differential media used to distinguish bacteria that are able to decarboxylate lysine and/or produce hydrogen sulfide from those that cannot. [1] This test is particularly useful for distinguishing different Gram-negative bacilli —especially among the Enterobacteriaceae .
It is a defined, selective and differential medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source. After citrate enters a cell through citrate permeases , citrate lyase cleaves it into acetate and oxaloacetate , which is further broken down into carbon dioxide and ...
Eosin methylene blue (EMB, also known as "Levine's formulation") is a selective and differential media used for the identification of Gram-negative bacteria, [1] specifically the Enterobacteriaceae. EMB inhibits the growth of most Gram-positive bacteria. EMB is often used to confirm the presence of coliforms in a sample.
Bile salts are the selective ingredient, while esculin is the differential component. Enterococcus hydrolyze esculin to products that react with ferric citrate in the medium to produce insoluble iron salts, resulting in the blackening of the medium. Test results must be interpreted in conjunction with gram stain morphology.
It is particularly useful for the isolation of organisms that cause bacillary dysentery, salmonella strains that cause food poisoning and Salmonella Paratyphi.It is not so selective for Salmonella Typhi.