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The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive and unfixed, outside the body, supravital stains are temporary in nature. [1] [2]
In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain , the core concept being that the cell ...
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
Brilliant cresyl blue is a supravital stain used for counting reticulocytes. [2] It is classified as an oxazine dye . N95 dust masks , eye shields, and gloves must all be worn when handling the chemical.
Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally, as was introduced by Leonor Michaelis in 1900. [2] The indicator Janus Green B changes colour according to the amount of oxygen present. [3] When oxygen is present, the indicator oxidizes to a blue colour.
Heinz body stain of feline blood, showing three distinct Heinz bodies. Heinz bodies appear as small round inclusions within the red cell body, though they are not visible when stained with Romanowsky dyes. They are visualized more clearly with supravital staining [5] [6] (e.g., with new methylene blue, crystal violet or bromocresol green).
Pappenheimer bodies (Peripheral Blood / May-Grünwald Giemsa and Prussian blue stain) Pappenheimer bodies are abnormal basophilic granules of iron found inside red blood cells on routine blood stain. [1] They are a type of inclusion body composed of ferritin aggregates, or mitochondria or phagosomes containing aggregated ferritin. They appear ...
The stain is an important histochemical stain used to demonstrate the distribution and amount of iron deposits in liver tissue, often in the form of a biopsy. [ 6 ] [ 7 ] Perls's procedure may be used to identify excess iron deposits such as hemosiderin deposits ( hemosiderosis ) and in conditions such as hereditary hemochromatosis . [ 8 ]