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2. Harbor Freight Tools - Wikipedia

   en.wikipedia.org/wiki/Harbor_Freight_Tools

   Harbor Freight Tools won a declassification of the class action; that is, the court found that all the individual situations were not similar enough to be judged as a single class, and that their claims would require an individual-by-individual inquiry, so the case could not be handled on a class basis.

3. CRISPR activation - Wikipedia

   en.wikipedia.org/wiki/CRISPR_activation

   Unlike traditional CRISPR-Cas9, which introduces double-strand breaks to edit genes, CRISPRa employs a modified, catalytically inactive Cas9 (dCas9) fused with transcriptional activators to target promoter or enhancer regions, thereby boosting gene transcription. This method allows for precise control of gene expression, making it a valuable ...

4. CRISPR - Wikipedia

   en.wikipedia.org/wiki/CRISPR

   Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within living organisms.

5. Eric Smidt - Wikipedia

   en.wikipedia.org/wiki/Eric_Smidt

   Harbor Freight Tools for Schools awards $1.5 million to skilled trades teachers and their schools annually. [ 10 ] Marking the opening of the 500th Harbor Freight Tools store near Chicago , Smidt announced the contribution of $100,000 and the donation of tools to Chicago Public Schools to support teachers and students in skilled trades learning ...

6. Coffee prices rise to nearly 50-year high due to weather ...

   www.aol.com/news/coffee-prices-rise-nearly-50...

   Coffee beans are hitting record high prices not seen in nearly 50 years after difficult growing seasons among some of the world's top producing regions. Earlier this week, the Wall Street Journal ...

7. Genome-wide CRISPR-Cas9 knockout screens - Wikipedia

   en.wikipedia.org/wiki/Genome-wide_CRISPR-Cas9...

   Targeted gene knockout using CRISPR/Cas9 requires the use of a delivery system to introduce the sgRNA and Cas9 into the cell. Although a number of different delivery systems are potentially available for CRISPR, [37] [38] genome-wide loss-of-function screens are predominantly carried out using third generation lentiviral vectors.