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Glucose oxidase is widely used for the determination of free glucose in body fluids (medical testing), in vegetal raw material, and in the food industry. It also has many applications in biotechnologies , typically enzyme assays for biochemistry including biosensors in nanotechnologies .
A colorless solid, it is an oxidized derivative of glucose. It is typically produced by the aerobic oxidation of glucose in the presence of the enzyme glucose oxidase. The conversion cogenerates hydrogen peroxide, which is often the key product of the enzyme: C 6 H 12 O 6 + O 2 → C 6 H 10 O 6 + H 2 O 2
Aspergillus niger is also cultured for the extraction of the enzyme, glucose oxidase, used in the design of glucose biosensors, due to its high affinity for β-D-glucose. [34] [35] In the food industry, A. niger is also cultured to isolate the enzyme fructosyltransferase to produce fructooligosaccharides (FOS). [36] FOS are used to manufacture ...
Monoamine oxidase (MAO) is an extensively studied flavoenzyme due to its biological importance with the catabolism of norepinephrine, serotonin and dopamine. MAO oxidizes primary, secondary and tertiary amines, which nonenzymatically hydrolyze from the imine to aldehyde or ketone .
He eventually found that these bacteria could only thrive adjacent to Aspergillus niger if glucose was not present. He eventually isolated the factor that caused the curious effect. The factor was glucose oxidase. In the presence of glucose, the glucose oxidase produced hydrogen peroxide, which killed off the bacteria. [1] [2] [3]
Photographer: Grant Webster, Food Stylist: Holly Dreesman, Prop Stylist: Joseph Wanek These bite-size dark chocolate almond clusters blend dark chocolate with nutty almonds for an easy snack or ...
Also arrange food evenly throughout a dish to help with cooking. If reheating sauce, soup or gravy, do so in a pan on the stove. Bring liquid dishes to a rolling boil, FoodSafety.gov advises.
A common example of a commercial biosensor is the blood glucose biosensor, which uses the enzyme glucose oxidase to break blood glucose down. In doing so it first oxidizes glucose and uses two electrons to reduce the FAD (a component of the enzyme) to FADH 2. This in turn is oxidized by the electrode in a number of steps.