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Raman spectroscopy offers several advantages for microscopic analysis. Since it is a light scattering technique, specimens do not need to be fixed or sectioned. Since it is a light scattering technique, specimens do not need to be fixed or sectioned.
Raman spectroscopy employs the Raman effect for substances analysis. The spectrum of the Raman-scattered light depends on the molecular constituents present and their state, allowing the spectrum to be used for material identification and analysis. Raman spectroscopy is used to analyze a wide range of materials, including gases, liquids, and ...
Typically, resonance Raman spectroscopy is performed in the same manner as ordinary Raman spectroscopy, using a single laser light source to excite the sample. The difference is the choice of the laser wavelength, which must be selected to match the energy of an electronic transition in the sample.
By using Raman microspectroscopy, in vivo time- and space-resolved Raman spectra of microscopic regions of samples can be measured. Sampling is non-destructive and water, media, and buffers typically do not interfere with the analysis. Consequently, in vivo time- and space-resolved Raman spectroscopy is suitable to examine proteins, cells and ...
The term surface enhanced Raman spectroscopy implies that it provides the same information that traditional Raman spectroscopy does, simply with a greatly enhanced signal. While the spectra of most SERS experiments are similar to the non-surface enhanced spectra, there are often differences in the number of modes present.
Stimulated Raman spectroscopy, also referred to as stimulated Raman scattering (SRS), is a form of spectroscopy employed in physics, chemistry, biology, and other fields. . The basic mechanism resembles that of spontaneous Raman spectroscopy: a pump photon, of the angular frequency , which is scattered by a molecule has some small probability of inducing some vibrational (or rotational ...
Raman spectroscopy combined with electrochemical techniques, makes Raman spectroelectrochemistry a powerful technique in the identification, characterization and quantification of molecules. The main advantage of Raman spectroelectrochemistry is that it is not limited to the selected solvent, and aqueous and organic solutions can be used.
The basic principle behind Raman spectroscopy differs from the MIR and NIR in that the x-axis of the Raman spectrum is measured as a function of energy shift (in cm −1) relative to the frequency of the laser used as the source of radiation. Briefly, the Raman spectrum arises from inelastic scattering of incident photons, which requires a ...
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