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In microscopy, NA generally refers to object-space numerical aperture unless otherwise noted. In microscopy, NA is important because it indicates the resolving power of a lens. The size of the finest detail that can be resolved (the resolution) is proportional to λ / 2NA , where λ is the wavelength of the light. A lens with a larger ...
Super-resolution microscopy. Super-resolution microscopy is a series of techniques in optical microscopy that allow such images to have resolutions higher than those imposed by the diffraction limit, [1][2] which is due to the diffraction of light. [3] Super-resolution imaging techniques rely on the near-field (photon-tunneling microscopy [4 ...
Phase-contrast imaging is a method of imaging that has a range of different applications. It measures differences in the refractive index of different materials to differentiate between structures under analysis. In conventional light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to ...
Photo-activated localization microscopy (PALM or FPALM) [1][2] and stochastic optical reconstruction microscopy (STORM) [3] are widefield (as opposed to point scanning techniques such as laser scanning confocal microscopy) fluorescence microscopy imaging methods that allow obtaining images with a resolution beyond the diffraction limit.
Macro photography. Macro photograph of a common yellow dung fly (Scathophaga stercoraria) made using a lens at its maximum 1:1 reproduction ratio, and an 18×24mm image sensor, the on-screen display of the photograph results in a greater than life-size image. Headshot of a dragonfly taken with a 100 mm macro lens coupled with a 50 mm lens in ...
October 22, 2024 at 4:00 AM. The 2024 Nikon Small World Photomicrography Competition, now in its 50th year, celebrates the beauty and science behind the smallest details of our world. Each year ...
Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction ...
Nikon launched the Optiphot and Labophot microscopes in the 1970's and established the CF Optical system. In the 1980's, they released 80 new products, including the inverted Diaphot microscope, which was used in IVF techniques. The introduction of the Eclipse range of infinity optics in the 1990's was a departure from traditional microscope ...