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  2. CRISPR - Wikipedia

    en.wikipedia.org/wiki/CRISPR

    Cas9 (or "CRISPR-associated protein 9") is an enzyme that uses CRISPR sequences as a guide to recognize and open up specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within living organisms.

  3. File:CRISPR Sterics.pdf - Wikipedia

    en.wikipedia.org/wiki/File:CRISPR_Sterics.pdf

    Original file (6,710 × 6,447 pixels, file size: 390 KB, MIME type: application/pdf) This is a file from the Wikimedia Commons . Information from its description page there is shown below.

  4. File:CRISPR effectors.pdf - Wikipedia

    en.wikipedia.org/wiki/File:CRISPR_effectors.pdf

    Original file (7,187 × 6,195 pixels, file size: 401 KB, MIME type: application/pdf) This is a file from the Wikimedia Commons . Information from its description page there is shown below.

  5. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    CRISPR gene editing (CRISPR, pronounced / ˈ k r ɪ s p ə r / (crisper), refers to a clustered regularly interspaced short palindromic repeats") is a genetic engineering technique in molecular biology by which the genomes of living organisms may be modified.

  6. Cas12a - Wikipedia

    en.wikipedia.org/wiki/Cas12a

    The CRISPR-Cas12a system consist of a Cas12a enzyme and a guide RNA that finds and positions the complex at the correct spot on the double helix to cleave target DNA. CRISPR-Cas12a systems activity has three stages: [3] Adaptation: Cas1 and Cas2 proteins facilitate the adaptation of small fragments of DNA into the CRISPR array.

  7. Cas9 - Wikipedia

    en.wikipedia.org/wiki/Cas9

    Cas9 (CRISPR associated protein 9, formerly called Cas5, Csn1, or Csx12) is a 160 kilodalton protein which plays a vital role in the immunological defense of certain bacteria against DNA viruses and plasmids, and is heavily utilized in genetic engineering applications.

  8. CRISPR-associated transposons - Wikipedia

    en.wikipedia.org/wiki/CRISPR-associated_transposons

    CRISPR-associated transposons have been harnessed for in vitro and in vivo gene editing at different targets, in different hosts, and with different payloads. All CAST components of the Tn6677 system from Vibrio cholerae have been combined into a single plasmid and confirmed to deliver up to 10kb transposons at near 100% efficiency. [16]

  9. Site-directed mutagenesis - Wikipedia

    en.wikipedia.org/wiki/Site-directed_mutagenesis

    Early attempts at mutagenesis using radiation or chemical mutagens were non-site-specific, generating random mutations. [2] Analogs of nucleotides and other chemicals were later used to generate localized point mutations, [3] examples of such chemicals are aminopurine, [4] nitrosoguanidine, [5] and bisulfite. [6]