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NIH-3T3 fibroblasts in cell culture.. 3T3 cells are several cell lines of mouse embryonic fibroblasts.The original 3T3 cell line (3T3-Swiss albino) was established in 1962 by two scientists then at the Department of Pathology in the New York University School of Medicine, George Todaro and Howard Green.
These cells developed into the commonly used cell line NIH 3T3. [4] MEFs treated by mitomycin or gamma rays (such treatment makes MEF stop mitosis) are widely used as feeder in embryonic stem cell culture because they can mimic the microenvironment in embryo. [5]
Differentiated adipocytes in a 3T3-L1 cell line stained with Oil Red O. 3T3-L1 is a sub clonal cell line derived from the original 3T3 Swiss albino cell line of 1962. The 3T3 original cell line was isolated from a mouse embryo and propagated for this specific line of 3T3 cells is used to study adipose tissue-related diseases and dysfunctions.
Fibroblasts can also migrate slowly over substratum as individual cells, again in contrast to epithelial cells. While epithelial cells form the lining of body structures, fibroblasts and related connective tissues sculpt the "bulk" of an organism. The life span of a fibroblast, as measured in chick embryos, is 57 ± 3 days. [4]
Cell culture or tissue culture is the process by which cells are grown under controlled ... NIH-3T3: NIH, 3-day transfer, inoculum 3 x 10 5 cells: Mouse: Embryo ...
The quality of G418 is not based on just the potency, but more on the selectivity defined by the killing curve of the sensitive cells vs the resistant cells. A good G418 product has the lowest LD 50 for sensitive cells (such as NIH 3T3) and the highest LD 50 (can be up to 5,000 μg/ml) for resistant cells (NIH 3T3 transfected with resistant ...
This gene encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This gene comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region. [5]
It also promotes the transition through G1 during the cell cycle and causes tumorgenic transformation of NIH/3T3 fibroblasts. [ 9 ] [ 10 ] The FGD1 gene is located on the short arm of the X-chromosome and is essential for normal mammalian embryonic development.
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