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The mitotic index is a measure of cellular proliferation. [1] It is defined as the percentage of cells undergoing mitosis in a given population of cells. Mitosis is the division of somatic cells into two daughter cells. Durations of the cell cycle and mitosis vary in different cell types. An elevated mitotic index indicates more cells are dividing.
For example, starfish regenerate lost arms through mitosis. Asexual reproduction: Some organisms produce genetically similar offspring through asexual reproduction. For example, the hydra reproduces asexually by budding. The cells at the surface of hydra undergo mitosis and form a mass called a bud. Mitosis continues in the cells of the bud and ...
Mitotic indexing is the oldest method of assessing proliferation and is determined by counting the number of mitotic figures (cells undergoing mitosis) through a light microscope on H&E stained sections. It is usually expressed as the number of cells per microscopic field.
mitotic index (MI) The proportion of cells within a sample which are undergoing mitosis at the time of observation, typically expressed as a percentage or as a value between 0 and 1. The number of cells dividing by mitosis at any given time can vary widely depending on organism, tissue, developmental stage, and culture media, among other ...
Mitotic cell division enables sexually reproducing organisms to develop from the one-celled zygote, which itself is produced by fusion of two gametes, each having been produced by meiotic cell division. [5] [6] After growth from the zygote to the adult, cell division by mitosis allows for continual construction and repair of the organism. [7]
A mitotic inhibitor (colchicine, colcemid) is then added to the culture. This stops cell division at mitosis which allows an increased yield of mitotic cells for analysis. The cells are then centrifuged and media and mitotic inhibitor are removed, and replaced with a hypotonic solution.
The mitotic spindle checkpoint verifies that all the chromosomes are aligned properly on the metaphase plate and prevents premature entry into anaphase. Chromosomes lined up on the metaphase plate. Two views with the metaphase plate rotated 60°. Stages of early mitosis in a vertebrate cell with micrographs of chromatids
Mitosis is the process of chromosome segregation and nuclear division that follows replication of the genetic material in eukaryotic cells. This process assures that each daughter nucleus receives a complete copy of the organism's genome .