Search results
Results from the WOW.Com Content Network
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes. [1] This, combined with the diffusion of oxygen from the top of the broth, produces a range of oxygen concentrations in the medium along its depth.
The following known results can be used to validate the proper composition of the medium: [1] Escherichia coli – ferments mannite, motile; Klebsiella sp. – ferments mannite, non-motile; Pseudomonas aeruginosa – does not ferment mannite, motile
A TSI slant is often used to distinguish nonfermenting Pseudomonas species from enteric pathogens in faecal specimens. [citation needed] When P. aeruginosa is isolated from a normally sterile site (blood, bone, deep collections), it is generally considered dangerous, and almost always requires treatment.
Cetrimide also enhances the production of Pseudomonas pigments such as pyocyanin and pyoverdine, which show a characteristic blue-green and yellow-green colour, respectively. [ 3 ] [ 4 ] Cetrimide agar is widely used in the examination of cosmetics, pharmaceuticals and clinical specimens to test for the presence of Pseudomonas aeruginosa .
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of Mycobacterium, notably Mycobacterium tuberculosis. [1] It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Get AOL Mail for FREE! Manage your email like never before with travel, photo & document views. Personalize your inbox with themes & tabs. You've Got Mail!
Eaton's agar is a type of agar media is used to grow Mycoplasma pneumoniae.. One recipe for the cultivation of M. pneumoniae (Eaton's agar) includes (): [1]. 70% Difco PPLO (pleuropneumonia-like organism) agar or broth base