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Giemsa's solution is a mixture of methylene blue, eosin, and Azure B.The stain is usually prepared from commercially available Giemsa powder. A thin film of the specimen on a microscope slide is fixed in pure methanol for 30 seconds, by immersing it or by putting a few drops of methanol on the slide.
Modern complete blood count analyzers can provide an automated white blood cell differential, but they have a limited ability to differentiate immature and abnormal cells, so manual examination of the blood smear is frequently indicated. [5] [6] Blood smear examination is the preferred diagnostic method for certain parasitic infections, such as ...
Whole blood with microfilaria worm, giemsa stain. L. loa worms have a simple structure consisting of a head (which lacks lips), a body, and a blunt tail. The outer body of the worm is composed of a cuticle with three main layers made up of collagen and other compounds which aid in protecting the nematodes while they are inside the digestive system of their host.
This stain develops varying colors for all cell structures (“Romanowsky-Giemsa effect) and thus was used in staining neutrophil polymorphs and cell nuclei. Common variants include Wright's stain, Jenner's stain, May-Grunwald stain, Leishman stain and Giemsa stain. All are used to examine blood or bone marrow samples.
These cells are often shades of grayish-blue. Polychromasia is usually a sign of bone marrow stress as well as immature red blood cells. 3 types are recognized, with types 1 and 2 being referred to as 'young red blood cells' and type 3 as 'old red blood cells'. Giemsa stain is used to distinguish all three types of blood smears. [1]
[1] [2] [3] The Diff-Quik procedure is based on a modification of the Wright-Giemsa stain pioneered by Harleco in the 1970s, [1] and has advantages over the routine Wright-Giemsa staining technique in that it reduces the 4-minute process into a much shorter operation and allows for selective increased eosinophilic or basophilic staining ...
The morphological characteristics of the hematopoietic cells are shown as seen in a Wright’s stain, May-Giemsa stain or May-Grünwald-Giemsa stain. Alternative names of certain cells are indicated between parentheses. Certain cells may have more than one characteristic appearance.
Pappenheimer bodies (Peripheral Blood / May-Grünwald Giemsa and Prussian blue stain) Pappenheimer bodies are abnormal basophilic granules of iron found inside red blood cells on routine blood stain. [1] They are a type of inclusion body composed of ferritin aggregates, or mitochondria or phagosomes containing aggregated ferritin. They appear ...