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In genetics, a strain is said to be auxotrophic if it carries a mutation that renders it unable to synthesize an essential compound. For example, a yeast mutant with an inactivated uracil synthesis pathway gene is a uracil auxotroph (e.g., if the yeast Orotidine 5'-phosphate decarboxylase gene is inactivated, the resultant strain is a uracil ...
URA3 is often used in yeast research as a "marker gene", that is, a gene to label chromosomes or plasmids. URA3 encodes Orotidine 5'-phosphate decarboxylase (ODCase) , which is an enzyme that catalyzes one reaction in the synthesis of pyrimidine ribonucleotides (a component of RNA ).
Next to the above-mentioned selection makers a few auxotrophic strains were generated to work with auxotrophic makers. The URA3 marker (URA3 blaster method) is an often-used strategy in uridine auxotrophic strains; however, studies have shown that differences in URA3 position in the genome can be involved in the pathogeny of C. albicans. [119]
Protease viability marker: One can look at protease function in specimens if they wish to target viability in cells; this practice in research is known as "Protease Viability Marker Assay Concept". The actions of protease cease once a cell dies, so a clear-cut line is drawn in determining cell viability when using this technique. [5]
Another method of selection is the use of certain auxotrophic markers that can compensate for an inability to metabolise certain amino acids, nucleotides, or sugars. This method requires the use of suitably mutated strains that are deficient in the synthesis or utility of a particular biomolecule, and the transformed cells are cultured in a ...
Selectable markers allow scientists to separate non-recombinant organisms (those which do not contain the selectable marker) from recombinant organisms (those which do); that is, a recombinant DNA molecule such as a plasmid expression vector is introduced into bacterial cells, and some bacteria are successfully transformed while some remain non-transformed.
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Yeast Metabolome Database; Content; Description: Metabolomics database: Data types captured: Yeast metabolite structures, metabolite descriptions, metabolite reactions, metabolite enzymes and transporters, yeast enzyme and transporter sequences, chemical properties, nomenclature, synonyms, chemical taxonomy, metabolite NMR spectra, metabolite GC-MS spectra, metabolite LC-MS spectra