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Viability PCR workflow. Viability PCR, also named v-PCR or vPCR, is an evolution of PCR.Through the use of a simple pre-treatment of the sample by the means of specific intercalating photo-reactive reagents it's possible to neutralize the DNA of dead cells.
DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample, typically a biological sample such as blood, saliva, or tissue. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components.
The removal of dead cells by neighboring phagocytic cells has been termed efferocytosis. [66] Dying cells that undergo the final stages of apoptosis display phagocytotic molecules, such as phosphatidylserine , on their cell surface. [ 67 ]
Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells ), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.
The morphometric method is a way to demonstrate cell death in the laboratory. Morphometric measurement provides the result of cell death as a volume, size, weight and length of tissue, organ and the whole organism that compares with before and after the occurrence of cell death. [2]
As described above, macrophages play a key role in removing dying or dead cells and cellular debris. Erythrocytes have a lifespan on average of 120 days and so are constantly being destroyed by macrophages in the spleen and liver. Macrophages will also engulf macromolecules, and so play a key role in the pharmacokinetics of parenteral irons.
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In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. [1]