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Mascot is a software search engine that uses mass spectrometry data to identify proteins from peptide sequence databases. [ 1 ] [ 2 ] Mascot is widely used by research facilities around the world. Mascot uses a probabilistic scoring algorithm for protein identification that was adapted from the MOWSE algorithm.
These methods determined the mass of peptides using mass spectrometry, and then used the mass to search protein databases to identify the proteins [3] [4] In 1999 a more complex program was released called Mascot that integrated three types of protein/database searches: peptide molecular weights, tandem mass spectrometry from one or more ...
The MOWSE algorithm was developed by Darryl Pappin at the Imperial Cancer Research Fund and Alan Bleasby at the SERC Daresbury Laboratory. [2] The probability-based MOWSE score formed the basis of development of Mascot, a proprietary software for protein identification from mass spectrometry data.
SEQUEST is a MS data analysis program used for protein identification. It correlates collections of tandem mass spectra to peptide sequences that have been generated from databases of protein sequences. [21] SIMS Open source: SIMS was designed to perform unrestricted PTM searches over tandem mass spectra. [22] SimTandem Freeware
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Links ORFs in complete genomes to protein 3D structures: Prokaryotes, Eukaryotes [16] geneid: Program to predict genes, exons, splice sites, and other signals along DNA sequences: Eukaryotes [17] GeneParser: Parse DNA sequences into introns and exons: Eukaryotes [18] GeneMark: Family of self-training gene prediction programs: Prokaryotes ...