Search results
Results from the WOW.Com Content Network
In physics and physical chemistry, time-resolved spectroscopy is the study of dynamic processes in materials or chemical compounds by means of spectroscopic techniques.Most often, processes are studied after the illumination of a material occurs, but in principle, the technique can be applied to any process that leads to a change in properties of a material.
This method (commonly referred to as time-resolved fluorometry or TRF) involves two fluorophores: a donor and an acceptor. Excitation of the donor fluorophore (in this case, the lanthanide ion complex) by an energy source (e.g. flash lamp or laser) produces an energy transfer to the acceptor fluorophore if they are within a given proximity to ...
When measuring fluorescence spectra, the wavelength of the excitation light is kept constant, preferably at a wavelength of high absorption, and the emission monochromator scans the spectrum. For measuring excitation spectra, the wavelength passing through the emission filter or monochromator is kept constant and the excitation monochromator is ...
Working principle of a streak camera. A streak camera is an instrument for measuring the variation in a pulse of light's intensity with time. They are used to measure the pulse duration of some ultrafast laser systems and for applications such as time-resolved spectroscopy and LIDAR.
For instance, the TADF exhibiting molecule ACRFLCN shows a strong sensitivity towards triplet oxygen making it an effective molecular oxygen sensor. [19] The fluorescein derivative DCF-MPYM has shown success in the field of bioimaging as its long lifetime allows time-resolved fluorescence imaging in living cells.
Jablonski diagram of FRET with typical timescales indicated. The black dashed line indicates a virtual photon.. Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). [1]
It measures the time difference between the excitation pulse and the photon detection, also called the time width (Δt). The fluorescence decay curve is obtained by plotting the measured time on the x-axis and the number of photons detected on the y-axis. However, it is difficult to simultaneously monitor multiple molecules.
Time-resolved photoluminescence (TRPL) is a method where the sample is excited with a light pulse and then the decay in photoluminescence with respect to time is measured. This technique is useful for measuring the minority carrier lifetime of III-V semiconductors like gallium arsenide .