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Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewing stained samples at high magnification, typically using bright-field or epi-fluorescence illumination.
Histologic specimen being placed on the stage of an optical microscope Human lung tissue stained with hematoxylin and eosin as seen under a microscope. Histology, [help 1] also known as microscopic anatomy or microanatomy, [1] is the branch of biology that studies the microscopic anatomy of biological tissues.
Anatomical terminology is used to describe microanatomical (or histological) structures. This helps describe precisely the structure, layout and position of an object, and minimises ambiguity. An internationally accepted lexicon is Terminologia Histologica.
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
In pathology, the Grocott–Gömöri's methenamine silver stain, abbreviated GMS, is a popular staining method in histology. The stain was originally named after György Gömöri, the Hungarian physician who developed the stain. It is used widely as a screen for fungal organisms. It is particularly useful in staining carbohydrates.
Phosphotungstic acid-haematoxylin staining demonstrating contraction band necrosis in an individual who had a myocardial infarction (heart attack). Phosphotungstic acid haematoxylin ( PTAH ) is a mix of haematoxylin with phosphotungstic acid , used in histology for staining .
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
Jenner's stain (methylene blue eosinate) is used in microscopy for staining blood smears. The stain is dark blue and results in very observable clearly stained nuclei. The stain is dark blue and results in very observable clearly stained nuclei.