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PlasMapper (Plasmid Mapper) [1] is a freely available web server that automatically generates and annotates high-quality circular plasmid maps. It is a particularly useful online service for molecular biologists wishing to generate plasmid maps without having to purchase or maintain expensive, commercial software.
A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes . In molecular biology , restriction maps are used as a reference to engineer plasmids or other relatively short pieces of DNA, and sometimes for longer genomic DNA.
Link alignment results to analysis tools (Primer design, Gel mobility and Maps, Plasmapper, siRNA design Epitope prediction), Save research logs, Create custom toolbars Accession number, GI number, PDB ID, FASTA , drag-drop from external URL from within the user interface
Molecular biology tools. Vector Database—A curated list of over 4,000 vector backbones, including relevant cloning information and bacterial growth conditions.. Sequence Analyzer—An Addgene software tool for creating plasmid maps from sequences with annotated features and restriction sites.
CGView was developed to address the specialized needs for visualizing and annotating circular genomes, such as bacterial, plasmid, chloroplast, mitochondrial DNA sequences. Once installed, the CGView program accepts a number of different file formats where feature data and rendering information can be XML file, a tab delimited file, or an NCBI ...
A pUC19 cloning vector showing the multiple cloning site sequence with restriction enzyme sites. A multiple cloning site (MCS), also called a polylinker, is a short segment of DNA which contains many (up to ~20) restriction sites—a standard feature of engineered plasmids. [1]
Vector map of pUC19. pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. [1] The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. [2]
There are two distinctive mapping approaches used in the field of genome mapping: genetic maps (also known as linkage maps) [7] and physical maps. [3] While both maps are a collection of genetic markers and gene loci, [8] genetic maps' distances are based on the genetic linkage information, while physical maps use actual physical distances usually measured in number of base pairs.