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In biochemical spectroscopy, information can be gathered about biological tissue by absorption and light scattering techniques. Light scattering spectroscopy is a type of reflectance spectroscopy that determines tissue structures by examining elastic scattering. [10] In such a case, it is the tissue that acts as a diffraction or dispersion ...
Spectrophotometry is an important technique used in many biochemical experiments that involve DNA, RNA, and protein isolation, enzyme kinetics and biochemical analyses. [16] Since samples in these applications are not readily available in large quantities, they are especially suited to be analyzed in this non-destructive technique.
In modern physics, the double-slit experiment demonstrates that light and matter can exhibit behavior of both classical particles and classical waves.This type of experiment was first performed by Thomas Young in 1801, as a demonstration of the wave behavior of visible light. [1]
By recording the attenuation of light for various wavelengths, an absorption spectrum can be obtained. In physics, absorption of electromagnetic radiation is how matter (typically electrons bound in atoms) takes up a photon's energy—and so transforms electromagnetic energy into internal energy of the absorber (for example, thermal energy). [1]
Molecules have various states referred to as energy levels.Fluorescence spectroscopy is primarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low energy state) of interest, and an excited electronic state of higher energy.
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay , that consists of a simple prepare and read method, without the requirement of separation or washing steps.
The mechanism of detection is based on the adsorbing molecules causing changes in the local index of refraction, changing the resonance conditions of the surface plasmon waves. The same principle is exploited in the recently developed competitive platform based on loss-less dielectric multilayers , supporting surface electromagnetic waves with ...
Medical physics, a branch of biophysics, is any application of physics to medicine or healthcare, ranging from radiology to microscopy and nanomedicine. For example, physicist Richard Feynman theorized about the future of nanomedicine. He wrote about the idea of a medical use for biological machines (see nanomachines).