Search results
Results from the WOW.Com Content Network
This example shows the general principle using visible light as a specific example. A white light source—emitting light of multiple wavelengths—is focused on a sample (the pairs of complementary colors are indicated by the yellow dotted lines). Upon striking the sample, photons that match the energy gap of the molecules present (green light ...
An example of spectroscopy: a prism analyses white light by dispersing it into its component colors. Spectroscopy is the field of study that measures and interprets electromagnetic spectra . [ 1 ] [ 2 ] In narrower contexts, spectroscopy is the precise study of color as generalized from visible light to all bands of the electromagnetic spectrum.
Biophysical chemistry is a physical science that uses the concepts of physics and physical chemistry for the study of biological systems. [1] The most common feature of the research in this subject is to seek an explanation of the various phenomena in biological systems in terms of either the molecules that make up the system or the supra-molecular structure of these systems. [2]
Microwave spectroscopy, for example, allows for the determination of bond lengths and angles with high precision. In addition, spectral measurements can be used to determine the accuracy of theoretical predictions. For example, the Lamb shift measured in the hydrogen atomic absorption spectrum was not expected to exist at the time it was measured.
Spectrophotometry is an important technique used in many biochemical experiments that involve DNA, RNA, and protein isolation, enzyme kinetics and biochemical analyses. [16] Since samples in these applications are not readily available in large quantities, they are especially suited to be analyzed in this non-destructive technique.
Photoexcitation is the first step in a photochemical process where the reactant is elevated to a state of higher energy, an excited state.The first law of photochemistry, known as the Grotthuss–Draper law (for chemists Theodor Grotthuss and John W. Draper), states that light must be absorbed by a chemical substance in order for a photochemical reaction to take place.
Fluorescence polarization immunoassay (FPIA) is a class of in vitro biochemical test used for rapid detection of antibody or antigen in sample. FPIA is a competitive homogenous assay , that consists of a simple prepare and read method, without the requirement of separation or washing steps.
For example, in photoacoustic spectroscopy, the photoacoustic signal is used to obtain the actual absorption of light in either opaque or transparent objects. It is useful for substances in extremely low concentrations, because very strong pulses of light from a laser can be used to increase sensitivity and very narrow wavelengths can be used ...