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Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
A colorimeter is a device used in colorimetry that measures the absorbance of particular wavelengths of light by a specific solution. [1] [2] It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.
The formed formazan dye is water-soluble, avoiding a final solubilization step. [ 9 ] Water-soluble tetrazolium salts are more recent alternatives to MTT: they were developed by introducing positive or negative charges and hydroxy groups to the phenyl ring of the tetrazolium salt, or better with sulfonate groups added directly or indirectly to ...
Forensic colorimetry, or forensic color analysis, is the examination of specimen color for purposes of forensic investigation.Typical specimens involved in color analyses include pigments, dyes, or other objects that are distinguishable by their intrinsic color.
Colorimetry; Colorimetry (chemical method) Calorimeter This page was last edited on 24 January 2025, at 00:05 (UTC). Text is available under the Creative Commons ...
Automated color measurement is a newer method of liquid color measurement. In this case the sample is contained in a test tube; the tube is inserted into the instrument, and the color properties of the liquid read out on a screen. This method can provide objective measurements, but is far more expensive than a set of color standards.
A camera or colorimeter is said to be colorimetric if it satisfies the Luther condition by Robert Luther (1868–1945) (also called the "Maxwell–Ives criterion"), [5] reducing observer metamerism color errors, if the product of the spectral responsivity of the photoreceptor and the spectral transmittance of the filters is a linear combination ...