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Colorimetric assays use reagents that undergo a measurable color change in the presence of the analyte. They are widely used in biochemistry to test for the presence of enzymes, specific compounds, antibodies, hormones and many more analytes. For example, para-Nitrophenylphosphate is converted into a yellow product by alkaline phosphatase enzyme.
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent.It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
A colorimeter is a device used in colorimetry that measures the absorbance of particular wavelengths of light by a specific solution. [1] [2] It is commonly used to determine the concentration of a known solute in a given solution by the application of the Beer–Lambert law, which states that the concentration of a solute is proportional to the absorbance.
Colorimetry is "the science and technology used to quantify and describe physically the human color perception". [1] It is similar to spectrophotometry , but is distinguished by its interest in reducing spectra to the physical correlates of color perception, most often the CIE 1931 XYZ color space tristimulus values and related quantities.
The Griess test is an analytical chemistry test which detects the presence of nitrite ion in solution.One of its most important uses is the determination of nitrite in drinking water.
Automated color measurement is a newer method of liquid color measurement. In this case the sample is contained in a test tube; the tube is inserted into the instrument, and the color properties of the liquid read out on a screen. This method can provide objective measurements, but is far more expensive than a set of color standards.
In colorimetry, the Munsell color system is a color space that specifies colors based on three properties of color: hue (basic color), value , and chroma (color intensity). It was created by Albert H. Munsell in the first decade of the 20th century and adopted by the United States Department of Agriculture (USDA) as the official color system ...
A camera or colorimeter is said to be colorimetric if it satisfies the Luther condition by Robert Luther (1868–1945) (also called the "Maxwell–Ives criterion"), [5] reducing observer metamerism color errors, if the product of the spectral responsivity of the photoreceptor and the spectral transmittance of the filters is a linear combination ...