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Dermatophyte test medium (DTM) is a specialized agar used in medical mycology. [ 1 ] [ 2 ] It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator.
A special agar called Dermatophyte Test Medium (DTM) has been formulated to grow and identify dermatophytes. [15] Without having to look at the colony, the hyphae, or macroconidia, one can identify the dermatophyte by a simple color test. The specimen (scraping from skin, nail, or hair) is embedded in the DTM culture medium.
Fungal spores may be viewed directly on hair shafts. This technique identifies a fungal infection in about 40%–70% of the infections, but cannot identify the species of dermatophyte. Culture test: This is the most effective, but also the most time-consuming, way to determine if ringworm is on a pet.
Yeast cells appear round or oval and budding forms may be seen. The KOH prep cannot identify the specific organism; the specimen can be submitted for fungal culture to identify the organism. A normal, or negative, KOH test shows no fungi (no dermatophytes or yeast). Dermatophytes or yeast seen on a KOH test indicate the person has a fungal ...
The hair perforation test, also known as an in vitro hair perforation test, is a laboratory test used to help distinguish the isolates of dermatophytes, such as Trichophyton mentagrophytes and its variants. [1]
The feathers are normally not affected by the dermatophyte, although some feather loss can occur. [1] Roosters and chicks tend to be more susceptible to the infection, with fighting cocks having the highest rates of M. gallinae dermatophytosis. [2] Although, it tends to resolve with treatment, the M. gallinae infection may become chronic. [1]
Microsporum gypseum is a soil-associated dermatophyte that occasionally is known to colonise and infect the upper dead layers of the skin of mammals. [1] The name refers to an asexual "form-taxon" that has been associated with four related biological species of fungi: the pathogenic taxa Arthroderma incurvatum, A. gypsea, A. fulva and the non-pathogenic saprotroph A. corniculata.
Tinea capitis may be difficult to distinguish from other skin diseases that cause scaling, such as psoriasis and seborrhoeic dermatitis; the basis for the diagnosis is positive microscopic examination and microbial culture of epilated hairs. [7]