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A calibration curve plot showing limit of detection (LOD), limit of quantification (LOQ), dynamic range, and limit of linearity (LOL).. In analytical chemistry, a calibration curve, also known as a standard curve, is a general method for determining the concentration of a substance in an unknown sample by comparing the unknown to a set of standard samples of known concentration. [1]
In summary, in order to find a standard curve, one must use varying concentrations of BSA (Bovine Serum Albumin) [2] in order to create a standard curve with concentration plotted on the x-axis and absorbance plotted on the y-axis. Only a narrow concentration of BSA is used (2-10 ug/mL) in order to create an accurate standard curve. [23]
These three equations show that the curves for CO 2 and HCO − 3 intersect at [H +] eq = K 1, and the curves for HCO − 3 and CO 2− 3 intersect at [H +] eq = K 2. Therefore, the values of K 1 and K 2 that were used to create a given Bjerrum plot can easily be found from that plot, by reading off the concentrations at these points of ...
Quantitative proteomics is an analytical chemistry technique for determining the amount of proteins in a sample. [1][2][3][4] The methods for protein identification are identical to those used in general (i.e. qualitative) proteomics, but include quantification as an additional dimension. Rather than just providing lists of proteins identified ...
Pharmacokinetics is based on mathematical modeling that places great emphasis on the relationship between drug plasma concentration and the time elapsed since the drug's administration. Pharmacokinetics is the study of how an organism affects the drug, whereas pharmacodynamics (PD) is the study of how the drug affects the organism.
The Standard addition method, often used in analytical chemistry, quantifies the analyte present in an unknown. This method is useful for analyzing complex samples where a matrix effect interferes with the analyte signal. In comparison to the calibration curve method, the standard addition method has the advantage of the matrices of the unknown ...
The calibration curve that does not use the internal standard method ignores the uncertainty between measurements. The coefficient of determination (R 2 ) for this plot is 0.9985. In the calibration curve that uses the internal standard, the y-axis is the ratio of the nickel signal to the yttrium signal.
Equilibrium unfolding. In biochemistry, equilibrium unfolding is the process of unfolding a protein or RNA molecule by gradually changing its environment, such as by changing the temperature or pressure, pH, adding chemical denaturants, or applying force as with an atomic force microscope tip. [1][2] If the equilibrium was maintained at all ...