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Fetal bovine serum (FBS) is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. This is due to it having a very low level of antibodies and containing more growth factors , allowing for versatility in many cell culture applications.
These “Synch” protocols may include: Select Synch, OvSynch, CoSynch, and Modified Select Synch [2] The synchronization of the estrous cycle is commonly used in different industries, such as Dairy and Beef cattle. Synchronization allows these industries to improve management and nutrition of the cattle, while also decreasing expenses.
Embryo transfer refers to a step in the process of assisted reproduction in which embryos are placed into the uterus of a female with the intent to establish a pregnancy.This technique - which is often used in connection with in vitro fertilization (IVF) - may be used in humans or in other animals, in which situations and goals may vary.
Embryomics is the identification, characterization and study of the diverse cell types which arise during embryogenesis, especially as this relates to the location and developmental history of cells in the embryo. Cell type may be determined according to several criteria: location in the developing embryo, gene expression as indicated by ...
In both PGS and PGD, individual cells from a pre-embryo, or preferably trophectoderm cells biopsied from a blastocyst, are analysed during the IVF process. Before the transfer of a pre-embryo back to a person's uterus, one or two cells are removed from the pre-embryos (8-cell stage), or preferably from a blastocyst. These cells are then ...
At the 16–cell stage the compacted embryo is called a morula. [14] [15] Once the embryo has divided into 16 cells, it begins to resemble a mulberry, hence the name morula (Latin, morus: mulberry). [16] Concomitantly, they develop an inside-out polarity that provides distinct characteristics and functions to their cell-cell and cell-medium ...
This showed it was possible to mix a sperm and an egg outside a women's reproductive system and create a healthy embryo. In 1959, Min Chueh Chang at the Worcester Foundation, proved fertilisation in vitro was capable of proceeding to a birth of a live rabbit. Chang's discovery was seminal, as it clearly demonstrated that oocytes fertilised in ...
A small publication of Mukherjee in 1978 [19] clearly shows that Mukherjee was on the right line of thinking much before anyone else had demonstrated the successful outcome of a pregnancy following the transfer of a 8-cell frozen-thawed embryo into human subjects transferring 8-cell cryopreserved embryos. [20] [21]