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Flow cytometry (FC) is a technique ... Light emitted from fluorophores are in a spectrum of wavelengths, ... This allows for the full spectra from each particle to be ...
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications [1] [2] including flow cytometry.First described in 1942, [3] FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (−N=C=S), replacing a hydrogen atom on the bottom ring of the structure.
J. Paul Robinson (ORCID 0000-0001-8383-3240) is an Australian/American educator, biologist, biomedical engineer, and expert in the applications of flow cytometry. [1] [2] He is a Distinguished Professor of Cytometry [3] in the Purdue University College of Veterinary Medicine, Department of Basic Medical Sciences, a professor of Biomedical Engineering in the Weldon School of Biomedical ...
Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. The rapid growth in the multidimensionality and throughput ...
Photoacoustic flow cytometry operates on the principle of the photoacoustic effect, whereby a laser in the visible spectrum produces a temperature rise and thus a thermal expansion. The thermal expansion equation with relation to laser intensity for a pulsating laser is described below.
Flow cytometry is a method in cell biology that employs the deflection of laser light a well as the excitation of fluorescent dyes to analyse various properties of a high number cells in a relatively short time. This category lists methods and tools used in flow cytometry.
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