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Transformation with Agrobacterium can be achieved in multiple ways. Protoplasts or alternatively leaf-discs can be incubated with the Agrobacterium and whole plants regenerated using plant tissue culture. In agroinfiltration the Agrobacterium may be injected directly into the leaf tissue of a plant. This method transforms only cells in ...
The main benefit of agroinfiltration when compared to the more traditional plant transformation is speed and convenience, although yields of the recombinant protein are generally also higher and more consistent. The first step is to introduce a gene of interest to a strain of Agrobacterium tumefaciens.
Agrobacterium-mediated T-DNA transfer is widely used as a tool in biotechnology.For more than two decades, Agrobacterium tumefaciens has been exploited for introducing genes into plants for basic research as well as for commercial production of transgenic crops. [11]
A number of methods are available to transfer DNA into plant cells. Some vector-mediated methods are: Agrobacterium-mediated transformation is the easiest and most simple plant transformation. Plant tissue (often leaves) are cut into small pieces, e.g. 10x10mm, and soaked for ten minutes in a fluid containing suspended Agrobacterium. The ...
In plants the DNA is often inserted using Agrobacterium-mediated recombination, [27] taking advantage of the Agrobacteriums T-DNA sequence that allows natural insertion of genetic material into plant cells. [28] Plant tissue are cut into small pieces and soaked in a fluid containing suspended Agrobacterium. The bacteria will attach to many of ...
Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants.The most commonly used plant transformation vectors are T-DNA binary vectors and are often replicated in both E. coli, a common lab bacterium, and Agrobacterium tumefaciens, a plant-virulent bacterium used to insert the recombinant DNA into plants.
In plants the DNA is often inserted using Agrobacterium-mediated recombination, [21] taking advantage of the Agrobacteriums T-DNA sequence that allows natural insertion of genetic material into plant cells. [22] Plant tissue are cut into small pieces and soaked in a fluid containing suspended Agrobacterium. The bacteria will attach to many of ...
Currently, there are several reports on standardisation of protocol for the Agrobacterium-mediated transformation. The effect of different parameters such as infection time, acetosyringone, DTT, and cysteine have been studied in soybean (Glycine max). [18] Possible plant compounds that initiate Agrobacterium to infect plant cells: [19]