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  2. Thin layer extraction - Wikipedia

    en.wikipedia.org/wiki/Thin_Layer_Extraction

    With a typical liquid mass diffusivity in the order of 10 −9 m 2 /s, [2] the characteristic time for diffusion through a 20 micron thick liquid layer is 0.4 s. Therefore, the thinness of both phases (organic and aqueous) causes a relatively "immediate" mass transfer of guest species from one phase to the other, which means that this process has a low mass transfer resistance.

  3. Aqueous two-phase system - Wikipedia

    en.wikipedia.org/wiki/Aqueous_two-phase_system

    It is a common observation that when oil and water are poured into the same container, they separate into two phases or layers, because they are immiscible.In general, aqueous (or water-based) solutions, being polar, are immiscible with non-polar organic solvents (cooking oil, chloroform, toluene, hexane etc.) and form a two-phase system.

  4. Liquid–liquid extraction - Wikipedia

    en.wikipedia.org/wiki/Liquid–liquid_extraction

    A process used to extract small amounts of organic compounds from water samples. [10] This process is done by injecting small amounts of an appropriate extraction solvent (C 2 Cl 4) and a disperser solvent (acetone) into the aqueous solution. The resulting solution is then centrifuged to separate the organic and aqueous layers. This process is ...

  5. Separatory funnel - Wikipedia

    en.wikipedia.org/wiki/Separatory_funnel

    The ether layer with a dissolved yellow compound is on top and an aqueous layer is at the bottom Apple , banana , carrot , and lettuce extraction using acetone , water, and dichloromethane . Lower layer is the organic layer.

  6. Work-up - Wikipedia

    en.wikipedia.org/wiki/Work-up

    The brine is used to remove any acid or water from the organic layer. In this example the organic layer is the product, which is a liquid at room temperature. The bottom aqueous layer is removed with a pipette and discarded. The top layer is transferred to an Erlenmeyer flask where it is treated with anhydrous sodium sulfate to remove any ...

  7. Extraction (chemistry) - Wikipedia

    en.wikipedia.org/wiki/Extraction_(chemistry)

    Laboratory-scale liquid-liquid extraction. Photograph of a separatory funnel in a laboratory scale extraction of 2 immiscible liquids: liquids are a diethyl ether upper phase, and a lower aqueous phase. Soxhlet extractor. Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. The distribution ...

  8. Phenol extraction - Wikipedia

    en.wikipedia.org/wiki/Phenol_extraction

    When suspended in a water-phenol solution, denatured proteins and unwanted cell components dissolve in the phenol, while polar nucleic acids dissolve in the water phase. [3] The solution may then be centrifuged to separate the phenol and water into distinct organic and aqueous phases. Purified nucleic acids can be precipitated from the aqueous ...

  9. Acid–base extraction - Wikipedia

    en.wikipedia.org/wiki/Acid–base_extraction

    The organic solvent used must be water-insoluble to observe phase separation and perform an acid-base extraction. [9] Three layers form in the separatory funnel. Often this is a result of insufficient mixing, and light stirring will solve the issue. [9] The boundary between the organic layer and aqueous layer is not observed.