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Rolling circle replication produces multiple copies of a single circular template. Rolling circle replication (RCR) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genome of viroids.
Rolling circle is a maneuver in which an aircraft rolls continuously while turning in a circle. This is arguably one of the most difficult maneuvers to perfect, since varying pitch and yaw corrections are necessary to keep the heading level while maintaining constant roll rate and turning radius. The standard rolling circle involves 1 roll at ...
Rolling-Circle Mechanism for Helitron transposition and gene acquisition in the concerted model. Helitron could be either autonomous or non-autonomous. One transposase molecule cleaves at the donor (by the first tyrosine (Y1) residue of the Rep protein) and target sites (by the second tyrosine (Y2) residue) and binds to the resulting 5' ends.
The circular fragments are copied by rolling circle replication resulting in many single-stranded copies of each fragment. The DNA copies concatenate head to tail in a long strand, and are compacted into a DNA nanoball. The nanoballs are then adsorbed onto a sequencing flow cell.
Helitron encoded proteins are composed of a rolling-circle (RC) replication initiator (Rep) and a DNA helicase (Hel) domain. The Rep domain is involved in the catalytic reactions for endonucleolytic cleavage, DNA transfer and ligation. In addition this domain contains three motifs. The first motif is necessary for DNA binding.
Rolling Loud, the world’s largest international hip-hop festival, returns to Miami’s Hard Rock Stadium this weekend for three days of music.. Future, Travis Scott and Playboi Carti are set to ...
A positive pitching movement is used at all points in the loop to draw the circle, so that the aeroplane canopy is pointing inwards. Both the inside and outside loop are sometimes casually referred to as a 'loop the loop'. Outside loop A vertical circle entered from straight and erect level flight, canopy pointing out of the loop. Loop can be ...
William Serrano-Franklin questions the investment that he and other Georgia State students are being asked to make in the team when the payoff, if any, will come long after they have graduated. “It’s like throwing your chips down on a roulette game and leaving before the ball stops rolling.” Dustin Chambers for The Huffington Post
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