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TBP is involved in DNA melting (double strand separation) by bending the DNA by 80° (the AT-rich sequence to which it binds facilitates easy melting). The TBP is an unusual protein in that it binds the minor groove using a β sheet. Another distinctive feature of TBP is a long string of glutamines in the N-terminus of the protein.
The TBP-associated factors (TAF) are proteins that associate with the TATA-binding protein in transcription initiation. It is a part of the transcription initiation factor TFIID multimeric protein complex .
TBP binds to the minor groove [15] of the TATA box via a region of antiparallel β sheets in the protein. [16] Three types of molecular interactions contribute to TBP binding to the TATA box: Four phenylalanine residues(Phe57, Phe74, Phe148, Phe165) on TBP bind to DNA and form kinks in the DNA, forcing the DNA minor groove open. [16] [17] [18]
TATA binding protein (TBP), a subunit of TFIID (the largest GTF) binds to the promoter (TATA box), creating a sharp bend in the promoter DNA. Then the TBP-TFIIA interactions recruit TFIIA to the promoter. TBP-TFIIB interactions recruit TFIIB to the promoter. RNA polymerase II and TFIIF assemble to form the Polymerase II complex.
The TBPL2 protein is also known as TBP-related factor 3 (TRF3) and TATA binding protein 2 (TBP2). The protein was independently discovered in three laboratories as a vertebrate-specific family member of TATA-binding protein (TBP). [6] [7] [8] Orthologs have been identified in mouse, frog (Xenopus tropicalis) and fish (Danio rerio). Whereas the ...
229906 Ensembl ENSG00000137947 ENSMUSG00000028271 UniProt Q00403 P62915 RefSeq (mRNA) NM_001514 NM_145546 RefSeq (protein) NP_001505 NP_663521 Location (UCSC) Chr 1: 88.85 – 88.89 Mb Chr 3: 142.47 – 142.49 Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II ...
But, the majority of TBP is present in the B-TF II D complex, which is composed of TBP and TAFII170 in a 1:1 ratio. [5] TF II D and B-TF II D are not equivalent, since transcription reactions utilizing TF II D are responsive to gene specific transcription factors such as SP1, while reactions reconstituted with B-TF II D are not.
Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is the basal transcription factor TFIID, which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.