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In genetics, a promoter is a sequence of DNA to which proteins bind to initiate transcription of a single RNA transcript from the DNA downstream of the promoter. The RNA transcript may encode a protein ( mRNA ), or can have a function in and of itself, such as tRNA or rRNA .
Further research at the late stage of thymocyte development reveals that distal Lck promoter with driven Cre will result in the distal lck gene promoter to drive Cre expression to be limited within innate-like T cells. There is a cell type specific function in innate-like T cells based on the distal lck promoter - driven Cre. [3]
The Pribnow box (also known as the Pribnow-Schaller box) is a sequence of TATAAT of six nucleotides (thymine, adenine, thymine, etc.) that is an essential part of a promoter site on DNA for transcription to occur in bacteria.
Promoter activity of the P-RM and P-R promoters vs RNA polymerase concentration in the enterobacteriophage lambda [1]. Promoter activity is a term that encompasses several meanings around the process of gene expression from regulatory sequences —promoters [2] and enhancers. [3]
The CAG promoter is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian expression vectors. [1] [2] CAG promoter was constructed in the lab of Dr Jun-ichi Miyazaki [3] [4] from the following sequences: (C) the cytomegalovirus (CMV) early enhancer element,
In molecular biology, insertional mutagenesis is the creation of mutations in DNA by the addition of one or more base pairs. Such insertional mutations can occur naturally, mediated by viruses or transposons , or can be artificially created for research purposes in the lab.
The initiator element (Inr) is the most common sequence found at the transcription start site of eukaryotic genes. It is a 17 bp element. Inr in humans was first explained and sequenced by two MIT biologists, Stephen T. Smale and David Baltimore in 1989. [2]
The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. [1] The goal of a well-designed expression vector is the efficient production of protein, and this may be achieved by the production of significant amount of stable ...