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Thin-layer chromatography (TLC) is a chromatography technique that separates components in non-volatile mixtures. [1] It is performed on a TLC plate made up of a non-reactive solid coated with a thin layer of adsorbent material. [2] This is called the stationary phase. [2]
Planar chromatography is a separation technique in which the stationary phase is present as or on a plane. The plane can be a paper, serving as such or impregnated by a substance as the stationary bed (paper chromatography) or a layer of solid particles spread on a support such as a glass plate (thin-layer chromatography).
High-performance thin-layer chromatography is first used to separate the lipids by physical and chemical characteristics, then transferred to a blotting matrix before the oligosaccharides are detected by a specific binding protein (i.e. antibodies or lectins).
Thin layer chromatography is used with reagent testing to separate substances before testing and prevent this "masking" effect. [4] Ehrlich reagent can only detect drugs with an indole moiety, but this is useful because drugs from the NBOMe class do not have an indole and are often sold as LSD which does. The Ehrlich reagent has an additional ...
Chromatography separates dissolved substances by different interaction with (i.e., travel through) a material. High-performance liquid chromatography (HPLC) Thin-layer chromatography (TLC) Countercurrent chromatography (CCC) Droplet countercurrent chromatography (DCC) Paper chromatography; Ion chromatography; Size-exclusion chromatography (SEC)
Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential absorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions.
The CRFs in thin layer chromatography characterize the equal-spreading of the spots. The ideal case, when the RF of the spots are uniformly distributed in <0,1> range (for example 0.25,0.5 and 0.75 for three solutes) should be characterized as the best situation possible.
Chiral thin-layer chromatography is a variant of liquid chromatography that is employed for the separation of enantiomers. It is necessary to use either a chiral stationary phase or; a chiral additive in the mobile phase.