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Antonie van Leeuwenhoek (1632–1723). The field of microscopy (optical microscopy) dates back to at least the 17th-century.Earlier microscopes, single lens magnifying glasses with limited magnification, date at least as far back as the wide spread use of lenses in eyeglasses in the 13th century [2] but more advanced compound microscopes first appeared in Europe around 1620 [3] [4] The ...
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Lasers are most widely used for more complex fluorescence microscopy techniques like confocal microscopy and total internal reflection fluorescence microscopy while xenon lamps, and mercury lamps, and LEDs with a dichroic excitation filter are commonly used for widefield epifluorescence microscopes.
1967: Erwin Wilhelm Müller adds time-of-flight spectroscopy to the field ion microscope, making the first atom probe and allowing the chemical identification of each individual atom. 1981: Gerd Binnig and Heinrich Rohrer develop the scanning tunneling microscope (STM). 1986: Gerd Binnig, Quate, and Gerber invent the atomic force microscope (AFM).
Bright-field microscopes have low apparent optical resolution due to the blur of out-of-focus material; Bright-field microscopes typically produce low contrast with most biological samples, as few absorb light to a great extent. Samples that are naturally colorless and transparent cannot be seen well, e.g. many types of mammalian cells.
Microscope slides are usually made of optical quality glass, such as soda lime glass or borosilicate glass, but specialty plastics are also used. Fused quartz slides are often used when ultraviolet transparency is important, e.g. in fluorescence microscopy. [3] [4] While plain slides are the most common, there are several specialized types.
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