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Major applications include blood smears, bone marrow aspirates, semen analysis and cytology of various body fluids including urine and cerebrospinal fluid. [7] [8] Microbiologic agents, such as bacteria and fungi, also appear more easily in Diff-Quik. [3] This is useful for the detection of for example Helicobacter pylori from gastric and ...
Gram stains are performed on body fluid or biopsy when infection is suspected. Gram stains yield results much more quickly than culturing , and are especially important when infection would make an important difference in the patient's treatment and prognosis; examples are cerebrospinal fluid for meningitis and synovial fluid for septic arthritis .
Once stained, they do not decolourize. The addition of heat during the staining process is a huge contributing factor. [15] Heat helps open the spore's membrane so the dye can enter. The main purpose of this stain is to show germination of bacterial spores.
Using aseptic technique, prepare and air dried heat fixed slide with the desired organism. Prepare a boiling water bath. Cover the slide with a piece of paper towel and place on staining rack over the water bath. Flood the paper towel on the slide with Malachite Green ( primary stain). Steam the slide for 5 to 7 minutes (mordant).
Using the innate circulatory system, the fixative is distributed throughout the entire body, and the tissue doesn't die until it is fixed. When this method is used, a drainage port must also be added somewhere in the circulatory system to account for the addition of the volume of the fixative and buffer, this is typically done in the right atrium .
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
An inoculation loop (also called a smear loop, inoculation wand or microstreaker) is a simple tool used mainly by microbiologists to pick up and transfer a small sample of microorganisms called inoculum from a microbial culture, e.g. for streaking on a culture plate. [1] [2] This process is called inoculation.
Examination of cells in body fluids was historically performed using a hemocytometer, a chamber designed for counting cells microscopically. [ 11 ] This technique was limited by poor discrimination between cell types (cells could only be classified as mononuclear or polymorphonuclear) and the low number of cells present in unconcentrated body ...