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[1] [2] Diagnoses and treatment of diseases such as leukemia and lymphoma often deal with hematopathology; techniques and technologies include flow cytometry studies [3] and immunohistochemistry. In the United States, hematopathology is a board-certified subspecialty by the American Board of Pathology. Board-eligible or board-certified ...
Hematologic disorders that may resemble CLL in their clinical presentation, behavior, and microscopic appearance include mantle cell lymphoma, marginal zone lymphoma, B cell prolymphocytic leukemia, and lymphoplasmacytic lymphoma. B cell prolymphocytic leukemia, a related, but more aggressive disorder, has cells with similar phenotype, but are ...
Immunophenotyping is a very common flow cytometry test in which fluorophore-conjugated antibodies are used as probes for staining target cells with high avidity and affinity. This technique allows rapid and easy phenotyping of each cell in a heterogeneous sample according to the presence or absence of a protein combination. [1]
Patients with this lymphoma commonly have an IgM gammaopathy, i.e. high blood levels of an IgM monoclonal protein. [6] Hairy cell leukemia: The monoclonal B-cells in this usually indolent CLL/SLL-like leukemia have a distinctive morphology and are CD5−, CD10−, CD19+, CD20+ (bright), CD23−, CD103+, CD200+, and complete Ig+. [9]
Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. [1] [2] [3] [4]In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
Micrograph showing Hodgkin's lymphoma, a type of B cell lymphoma that is usually considered separate from other B cell lymphomas. Field stain. CT scan of primary B cell lymphoma in the left ilium, as diffuse cortical and trabecular thickening of the hemipelvis, mimicking Paget's disease. [2] There are numerous kinds of lymphomas involving B cells.
After lymphoma is diagnosed, a variety of tests may be carried out to look for specific features characteristic of different types of lymphoma. These include: Immunophenotyping; Flow cytometry; Fluorescence in situ hybridization testing
The applicability of IT-MTX varies across lymphoma subtypes, and caution is warranted in extrapolating findings from DLBCL to other lymphoma entities such as Burkitt lymphoma. [3] Moreover, the shifting understanding of disease biology, including the impact of genetic alterations and tumor microenvironment , further complicates decision-making ...
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