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An endospore stain of the cell Bacillus subtilis showing endospores as green and the vegetative cell as red Phase-bright endospores of Paenibacillus alvei imaged with phase-contrast microscopy An endospore is a dormant , tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota .
Endospore stain on Bacillus subtilis.The spore is stained green and the vegetative cell is stained a pinkish red color. Endospore staining is a technique used in bacteriology to identify the presence of endospores in a bacterial sample. [1]
There is debate as to whether endospory or heterospory evolved first. Some debate centers upon the requirement of endospory to develop before heterospory. [2] Endospory is assumed to follow heterospory but it has been suggested that without endospory, early plant species dependency on water fertilization and environmental impacts on gametophytic gene expression would have reduced the chances ...
Perhaps the best known bacterial adaptation to stress is the formation of endospores. Endospores are bacterial survival structures that are highly resistant to many different types of chemical and environmental stresses and therefore enable the survival of bacteria in environments that would be lethal for these cells in their normal vegetative ...
Fig1. The sporulation process of Bacillus subtilis. Bacillus subtilis is a rod-shaped, Gram-positive bacteria that is naturally found in soil and vegetation, and is known for its ability to form a small, tough, protective and metabolically dormant endospore.
Also important in the generation of the term, is the delineation of endospores and microbial cysts as different forms of cryptobiosis or dormancy. Endospores exhibit more extreme isolation from their environment in terms of cell wall thickness, impermeability to substrates, and presence of dipicolinic acid , a compound known to confer ...
In vivo staining (also called vital staining or intravital staining) is the process of dyeing living tissues. By causing certain cells or structures to take on contrasting colours, their form or position within a cell or tissue can be readily seen and studied.
Clostridium endospores have a distinct bowling pin or bottle shape, distinguishing them from other bacterial endospores, which are usually ovoid in shape. [citation needed] The Schaeffer–Fulton stain (0.5% malachite green in water) can be used to distinguish endospores of Bacillus and Clostridium from other microorganisms. [12]