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  2. Phage display - Wikipedia

    en.wikipedia.org/wiki/Phage_display

    Antibody phage display was later used by Carlos F. Barbas at The Scripps Research Institute to create synthetic human antibody libraries, a principle first patented in 1990 by Breitling and coworkers (Patent CA 2035384), thereby allowing human antibodies to be created in vitro from synthetic diversity elements.

  3. Affinity maturation - Wikipedia

    en.wikipedia.org/wiki/Affinity_maturation

    In immunology, affinity maturation is the process by which T FH cell-activated B cells produce antibodies with increased affinity for antigen during the course of an immune response. With repeated exposures to the same antigen, a host will produce antibodies of successively greater affinities .

  4. John McCafferty - Wikipedia

    en.wikipedia.org/wiki/John_McCafferty

    John McCafferty is a British scientist, one of the founders of Cambridge Antibody Technology alongside Sir Gregory Winter and David Chiswell. He is well known as one of the inventors of scFv antibody fragment phage display, [1] a technology that revolutionised the monoclonal antibody drug discovery.

  5. DNA-encoded chemical library - Wikipedia

    en.wikipedia.org/wiki/DNA-encoded_chemical_library

    DNA encoded chemical libraries bear resemblance to biological display technologies such as antibody phage display technology, yeast display, mRNA display and aptamer SELEX. In antibody phage display, antibodies are physically linked to phage particles that bear the gene coding for the attached antibody, which is equivalent to a physical linkage ...

  6. Humanized antibody - Wikipedia

    en.wikipedia.org/wiki/Humanized_antibody

    These employ the selective principles of specific antibody production but exploit micro-organisms (as in phage display) or even cell free extracts (as in ribosome display). These systems rely on the creation of antibody gene "libraries" which can be wholly derived from human RNA isolated from peripheral blood.

  7. Cambridge Antibody Technology - Wikipedia

    en.wikipedia.org/wiki/Cambridge_Antibody_Technology

    Cambridge Antibody Technology Group Plc, (commonly referred to as CAT) was a biotechnology company headquartered in Cambridge, England, United Kingdom.Its core focus was on antibody therapeutics, primarily using Phage Display and Ribosome Display technology.

  8. Directed evolution - Wikipedia

    en.wikipedia.org/wiki/Directed_evolution

    Early phage display techniques in the 1980s ... to be set for sorting a variant or population of variants ... therapeutic antibodies (Affinity maturation) ...

  9. Bacterial display - Wikipedia

    en.wikipedia.org/wiki/Bacterial_display

    Bacterial display (or bacteria display or bacterial surface display) is a protein engineering technique used for in vitro protein evolution. Libraries of polypeptides displayed on the surface of bacteria can be screened using flow cytometry or iterative selection procedures (biopanning).