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A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours. The presence of some coliforms indicate fecal contamination. The term "IMViC" is an acronym for each of these tests. "I" is for indole test; "M" is for methyl red test; "V" is for Voges-Proskauer test, and "C" is for citrate ...
The fecal coliform assay is intended to be an indicator of fecal contamination; more specifically of E. coli which is an indicator microorganism for other pathogens that may be present in feces. Presence of fecal coliforms in water may not be directly harmful, and do not necessarily indicate the presence of feces. [1]
Consequently, the National Technical Advisory Committee in the US recommended the following standard for recreational waters in 1968: 10 percent of total samples during any 30-day period should not exceed 400 fecal coliforms/100 mL or a log mean of 200/100 mL (based on a minimum of 5 samples taken over not more than a 30-day period).
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Coliform bacteria are defined as either motile or non-motile Gram-negative non-spore forming bacilli that possess β-galactosidase to produce acids and gases under their optimal growth temperature of 35–37 °C. [1]
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Fecal coliform, at concentrations of 2,875 units/100 milliliters, was found in a pipe that drains into Laurel Creek, which winds through the city’s 26-acre Hunt Woods Park, where dogs bathe in ...
In a sample, E. coli, which is citrate-negative, can be distinguished from non-fecal, citrate-positive coliforms that are often found in water, soil, and on plants using Simmons’ agar. Additionally, Simmons’ agar is commonly used as part of the IMViC tests to identify coliforms. [4]
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