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Average HER2 copy number per cell ≥4.0 HER2 positive Additional work-up required <4.0 Additional work-up required HER2 negative
In cancer cells the HER2 protein can be expressed up to 100 times more than in normal cells (2 million versus 20,000 per cell). ... to estimate HER2 copy number from ...
Many biotechnology applications utilize mutated plasmids that replicate to high copy number. For example, pBR322 is a medium copy number plasmid (~20 copies/cell) from which several high copy number cloning vectors (>100 copies/cell) have been derived by mutagenesis, such as the well known pUC series. [1] This delivers the convenience of high ...
Gene dosage is the number of copies of a particular gene present in a genome. [1] Gene dosage is related to the amount of gene product (proteins or functional RNAs) the cell is able to express. Since a gene acts as a template, the number of templates in the cell contributes to the amount of gene product able to be produced.
Generally, each molecule of plasmid diffuses randomly, so the probability of having a plasmid-less daughter cell is 2 1−N, where N is the number of copies. For instance, if there are 2 copies of a plasmid in a cell, there is 50% chance of having one plasmid-less daughter cell. However, high-copy number plasmids have a cost for the hosting cell.
News of the “Phantom of Heilbronn” first spread across Germany – and throughout Europe – in 2007, when a 22-year-old police officer in the southern city of Heilbronn was murdered and DNA ...
After over 100 people were killed in a South Korea plane accident, here are the worst accidents and crashes in aviation history.
The dome at the US Capitol is shrouded in fog earlier this month in Washington. (The Washington Post via Getty Images) (The Washington Post via Getty Images)
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