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where N is the uncorrected f-number, NA i is the image-space numerical aperture of the lens, | | is the absolute value of the lens's magnification for an object a particular distance away, and P is the pupil magnification. Since the pupil magnification is seldom known it is often assumed to be 1, which is the correct value for all symmetric lenses.
With any telescope, microscope or lens, a maximum magnification exists beyond which the image looks bigger but shows no more detail. It occurs when the finest detail the instrument can resolve is magnified to match the finest detail the eye can see. Magnification beyond this maximum is sometimes called "empty magnification".
More generally, for situations where it is possible to raise a telescope's magnification high enough to make the sky background effectively black, the limiting magnitude is approximated by = + (/) where and are as stated above, is the observer's pupil diameter in centimetres, and is the telescope transmittance (e.g. 0.75 for a typical ...
For a telescope, the diameter of the exit pupil can be calculated by dividing the focal length of the eyepiece by the focal ratio (f-number) of the telescope. In all but the cheapest telescopes, the eyepieces are interchangeable, and for this reason, the magnification is not written on the scope, as it will change with different eyepieces.
Here NA is the numerical aperture, is half the included angle of the lens, which depends on the diameter of the lens and its focal length, is the refractive index of the medium between the lens and the specimen, and is the wavelength of light illuminating or emanating from (in the case of fluorescence microscopy) the sample.
The eye relief of an optical instrument (such as a telescope, a microscope, or binoculars) is the distance from the last surface of an eyepiece within which the user's eye can obtain the full viewing angle. If a viewer's eye is outside this distance, a reduced field of view will be obtained.
In microscopy, NA is important because it indicates the resolving power of a lens. The size of the finest detail that can be resolved (the resolution) is proportional to λ / 2NA , where λ is the wavelength of the light. A lens with a larger numerical aperture will be able to visualize finer details than a lens with a smaller numerical ...
As with binoculars and telescopes, monoculars are primarily defined by two parameters: magnification and objective lens diameter, for example, 8×30 where 8 is the magnification and 30 is the objective lens diameter in mm (this is the lens furthest from the eye). An 8× magnification makes the distant object appear to be 8 times larger at the eye.
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