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The absorbance of the blue solution can be measured with a colorimeter and the concentration of phosphate in the original solution can be calculated. Alternatively, a direct (but approximate) reading of phosphate concentration can be obtained by using a Lovibond comparator. This method for phosphate determination is known as Denigés' method ...
The enzyme alkaline phosphatase (ALP, alkaline phenyl phosphatase) is a phosphatase with the physiological role of dephosphorylating compounds. The enzyme is found across a multitude of organisms, prokaryotes and eukaryotes alike, with the same general function, but in different structural forms suitable to the environment they function in. Alkaline phosphatase is found in the periplasmic ...
In chemistry, a phosphate is an anion, salt, functional group or ester derived from a phosphoric acid. It most commonly means orthophosphate, a derivative of orthophosphoric acid, a.k.a. phosphoric acid H 3 PO 4. The phosphate or orthophosphate ion [PO 4] 3− is derived from phosphoric acid by the removal of three protons H +.
The term phosphate is also used in organic chemistry for the functional groups that result when one or more of the hydrogens are replaced by bonds to other groups. These acids, together with their salts and esters , include some of the best-known compounds of phosphorus, of high importance in biochemistry , mineralogy , agriculture , pharmacy ...
5' Nucleotidase (5NT) is a glycoprotein found throughout the body, in the cytoplasmic membrane, catalyzing the conversion to inorganic phosphates from nucleoside-5-phosphate. Its level is raised in conditions such as obstructive jaundice, parenchymal liver disease, liver metastases, and bone disease.
Sodium hexametaphosphate is the alkali salt of one of the series of polymetaphosphoric acids (acids formed by the polymerization of phosphate groups). [14] Hexametaphosphoric acid was first made in 1825 by the German chemist Johann Frederich Philipp Engelhart (1797-1853). [15]
Trisodium phosphate (TSP) is an inorganic compound with the chemical formula Na 3 P O 4. It is a white, granular or crystalline solid, highly soluble in water, producing an alkaline solution. TSP is used as a cleaning agent , builder , lubricant , food additive , stain remover , and degreaser .
It is commonly used in the determination of phosphate ion content. [1] [2] The reagent used is ammonium molybdovanadate with the addition of 70% perchloric acid (sulfuric acid is also known to be used). [citation needed] It is used for purposes such as the analysis of wine, canned fruits and other fruit-based products such as jams and syrups ...
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