Search results
Results from the WOW.Com Content Network
The Purnell equation is an equation used in analytical chemistry to calculate the resolution R s between two peaks in a chromatogram. [1] [2] = (′ + ′) where R s is the resolution between the two peaks N 2 is the plate number of the second peak α is the separation factor between the two peaks k ' 2 is the retention factor of the second peak.
The response factor can be expressed on a molar, volume or mass [1] basis. Where the true amount of sample and standard are equal: = where A is the signal (e.g. peak area) and the subscript i indicates the sample and the subscript st indicates the standard. [2]
Two resolved peaks in a chromatogram. The theoretical plate height is given by = where L is the column length and N the number of theoretical plates. [5] The relation between plate number and peak width at the base is given by = ().
OpenChrom is an open source software for the analysis and visualization of mass spectrometric and chromatographic data. [4] Its focus is to handle native data files from several mass spectrometry systems (e.g. GC/MS, LC/MS, Py-GC/MS, HPLC-MS), vendors like Agilent Technologies, Varian, Shimadzu, Thermo Fisher, PerkinElmer and others.
The fundamental resolution equation is derived as follows: For two closely spaced peaks, ω 1 = ω 2, and σ 1 = σ 2. so R s = (t r2 - t r1)/ω 2 = (t r2 - t r1)/4σ 2. Where t r1 and t r2 are the retention times of two separate peaks. Since N = [(t r2)/σ 2] 2, then σ = t r2 / N 1/2. Using substitution, R s = N 1/2 [(t r2 - t r1)/4t r2] = (N ...
PeakFit is an automated nonlinear peak separation and analysis software package for scientists performing spectroscopy, chromatography and electrophoresis.. PeakFit automatically finds and fits up to 100 peaks to a data set, at a time, enabling users to characterize peaks and find the best equation that fits their data.
Chromatography software is called also Chromatography Data System. [1] It is located in the data station of the modern liquid, gas or supercritical fluid chromatographic systems. This is a dedicated software connected to an hardware interface within the chromatographic system, which serves as a central hub for collecting, analyzing, and ...
A high value for resolution corresponding to good separation of peaks is similar to the convention used with chromatography separations, [13] although it is important to note that the definitions are not the same. [14] High resolution indicating better peak separation is also used in ion mobility spectrometry. [15]