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The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Movat's stain is a pentachrome stain originally developed by Henry Zoltan Movat (1923–1995), a Hungarian-Canadian Pathologist in Toronto [1] in 1955 to highlight the various constituents of connective tissue, especially cardiovascular tissue, by five colors in a single stained slide. [2]
Furthermore, areolar tissue is the same as loose connective tissue, adipose tissue is a subset of specialized connective tissue, and reticular tissue is the presence of reticular fibers and reticular cells together forming the stroma of hemopoietic tissue (specifically the red bone marrow) and lymphatic tissue organs (lymph nodes and spleen but ...
Similar to the frozen section procedure employed in medicine, cryosectioning is a method to rapidly freeze, cut, and mount sections of tissue for histology. The tissue is usually sectioned on a cryostat or freezing microtome. [12] The frozen sections are mounted on a glass slide and may be stained to enhance the contrast between different tissues.
Microscopic view of a histologic specimen of human lung tissue stained with hematoxylin and eosin. Haematoxylin and eosin staining is frequently used in histology to examine thin tissue sections. [10] Haematoxylin stains cell nuclei blue, while eosin stains cytoplasm, connective tissue and other extracellular substances pink or red. [10]
Phosphotungstic acid haematoxylin (PTAH) is a mix of haematoxylin with phosphotungstic acid, used in histology for staining. It stains some tissue in contrasting colors in a way similar to haematoxylin and eosin stain, as phosphotungstic acid binds to tissue proteins.
The lamina propria is a loose connective tissue, hence it is not as fibrous as the underlying connective tissue of the submucosa. [4] [self-published source?] The connective tissue and architecture of the lamina propria is very compressible and elastic, this can be seen in organs that require expansion such as the bladder. [5]
Connective tissue is one of the four primary types of animal tissue, a group of cells that are similar in structure, along with epithelial tissue, muscle tissue, and nervous tissue. [1] It develops mostly from the mesenchyme , derived from the mesoderm , the middle embryonic germ layer . [ 2 ]
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