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  2. Oil immersion - Wikipedia

    en.wikipedia.org/wiki/Oil_immersion

    Oil immersion objective lenses look superficially identical to non-oil immersion lenses. In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical ...

  3. Objective (optics) - Wikipedia

    en.wikipedia.org/wiki/Objective_(optics)

    Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...

  4. High-power field - Wikipedia

    en.wikipedia.org/wiki/High-power_field

    The area provides a reference unit, for example in reference ranges for urine tests. [3]Used for grading of soft tissue tumors: Grading, usually on a scale of I to III, is based on the degree of differentiation, the average number of mitoses per high-power field, cellularity, pleomorphism, and an estimate of the extent of necrosis (presumably a reflection of rate of growth).

  5. Dark-field microscopy - Wikipedia

    en.wikipedia.org/wiki/Dark-field_microscopy

    In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...

  6. Optical microscope - Wikipedia

    en.wikipedia.org/wiki/Optical_microscope

    The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.

  7. Magnification - Wikipedia

    en.wikipedia.org/wiki/Magnification

    A telescope, which uses its large objective lens or primary mirror to create an image of a distant object and then allows the user to examine the image closely with a smaller eyepiece lens, thus making the object look larger. A microscope, which makes a small object appear as a much larger image at a comfortable distance for viewing. A ...

  8. Stereo microscope - Wikipedia

    en.wikipedia.org/wiki/Stereo_microscope

    A - Objective B - Galilean telescopes (rotating objectives) C - Zoom control D - Internal objective E - Prism F - Relay lens G - Reticle H - Eyepiece The stereo , stereoscopic or dissecting microscope is an optical microscope variant designed for low magnification observation of a sample, typically using light reflected from the surface of an ...

  9. Microscopic scale - Wikipedia

    en.wikipedia.org/wiki/Microscopic_scale

    It is important to note that the resolution between two objects varies from individual to individual, [9] but the strength of the objective lenses can be quantified. [11] In the 1660s, Antonie van Leeuwenhoek devised a simple microscope utilising a single spherical lens mounted between two thin brass plates. Depending on the quality of the lens ...