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Pharmacology of estrone Mean vaginal cornification (grade) with a single subcutaneous injection of estrone or estradiol in aqueous suspension in around 10 women each. [ 1 ] [ 2 ] Vaginal cornification grade is percentage cornification of vaginal epithelial cells and is 1 (+) = 10–25%, 2 (++) = 25–50%, and 3 (+++) = 50–75%.