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This list of protein subcellular localisation prediction tools includes software, databases, and web services that are used for protein subcellular localization prediction. Some tools are included that are commonly used to infer location through predicted structural properties, such as signal peptide or transmembrane helices , and these tools ...
Zymoblot is the fastest available microtechnique to detect gene expression or enzyme activity in any biological specimen. The technique was invented by Professor Elsayed Elsayed Wagih in collaboration with Professor Jacqueline Fletcher of the Department of Plant Pathology, Noble Research Centre , Oklahoma State University , US in 1993.
The phagocytosis process took place in secondary lysosomes and the autophagy and heterophagy controlled the dead cell by acid hydrolysis activity. The techniques used to explained this is by the detection of (6-3H)-thymidine and acid phosphates' activity in cryostat.
A lysosome (/ ˈ l aɪ s ə ˌ s oʊ m /) is a single membrane-bound organelle found in many animal cells. [1] [2] They are spherical vesicles that contain hydrolytic enzymes that digest many kinds of biomolecules. A lysosome has a specific composition, of both its membrane proteins and its lumenal proteins.
A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the western blot method, with the exception that the proteins to be detected are not first separated by electrophoresis. Instead, the sample is applied directly on a membrane in a single spot, and the blotting procedure is ...
1.2.1 Lysosome and proteasome. 1.2.2 Rate ... Proteolysis can also be used as an analytical tool for studying proteins in the laboratory, and it may also be used in ...
The process of phagocytosis showing phagolysosome formation. Lysosome(shown in green) fuses with phagosome to form a phagolysosome. Membrane fusion of the phagosome and lysosome is regulated by the Rab5 protein, [1] a G protein that allows the exchange of material between these two organelles but prevents complete fusion of their membranes. [1]
The detection of horseradish peroxidase by enzymatic chemiluminescence (ECL) is a common method of detecting antibodies in western blotting. Another example is the enzyme luciferase , this is found in fireflies and naturally produces light from its substrate luciferin.