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The annealing temperature during a polymerase chain reaction determines the specificity of primer annealing. The melting point of the primer sets the upper limit on annealing temperature. At temperatures just below this point, only very specific base pairing between the primer and the template will occur.
Pfu DNA polymerase is an enzyme found in the hyperthermophilic archaeon Pyrococcus furiosus, where it functions to copy the organism's DNA during cell division (thermostable DNA polymerase).
In touchdown PCR, the annealing temperature is gradually decreased in later cycles. The annealing temperature in the early cycles is usually 3–5 °C above the standard T m of the primers used, while in the later cycles it is a similar amount below the T m. The initial higher annealing temperature leads to greater specificity for primer ...
Download QR code; Print/export Download as PDF; ... move to sidebar hide. Annealing temperature may refer to: Annealing (glass) Annealing (metallurgy) ...
This temperature is affected by the rotational speed of the pulleys, the ambient temperature, and the voltage applied. Where t {\displaystyle t} is the temperature of the wire, K {\displaystyle K} is a constant, V {\displaystyle V} is the voltage applied, r {\displaystyle r} is the number of rotations of the pulleys per minute, and t a ...
Academic Torrents [1] [2] [3] [4] [5] [6] is a website which enables the sharing of research data using the BitTorrent protocol. The site was founded in November 2013 ...
Adaptive simulated annealing (ASA) is a variant of simulated annealing (SA) algorithm in which the algorithm parameters that control temperature schedule and random step selection are automatically adjusted according to algorithm progress. This makes the algorithm more efficient and less sensitive to user defined parameters than canonical SA.
The technique of simulated annealing, by which an existing MSA produced by another method is refined by a series of rearrangements designed to find better regions of alignment space than the one the input alignment already occupies. Like the genetic algorithm method, simulated annealing maximizes an objective function like the sum-of-pairs ...