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The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
In cryo-sectioning, frozen blocks of cells or tissue are sectioned into thin samples with a cryo-microtome. [11] In FIB-milling, plunge-frozen samples are exposed to a focused beam of ions, typically gallium , that precisely whittle away material from the top and bottom of a sample, leaving a thin lamella suitable for cryoET imaging.
Electric cryo chambers use refrigeration as a cooling agent providing a safe, breathable environment, not exposing the client to any dangerous gases such as nitrogen. Cryotherapy is a specific type of low-temperature treatment used to reduce inflammation and its associated pain.
A cryostat (from cryo meaning cold and stat meaning stable) is a device used to maintain low cryogenic temperatures of samples or devices mounted within the cryostat. Low temperatures may be maintained within a cryostat by using various refrigeration methods, most commonly using cryogenic fluid bath such as liquid helium . [ 1 ]
Cryo ultramicrotome In 1952 Humberto Fernandez Morán introduced cryo ultramicrotomy , which is a similar technique but done at freezing temperatures between −20 and −150°C. Cryo ultramicrotomy can be used to cut ultra-thin frozen biological specimens.
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections ...
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Cryofixation is a technique for fixation or stabilisation of biological materials as the first step in specimen preparation for the electron microscopy and cryo-electron microscopy. [1] Typical specimens for cryofixation include small samples of plant or animal tissue , cell suspensions of microorganisms or cultured cells , suspensions of ...