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The frozen section procedure as practiced today in medical laboratories is based on the description by Dr Louis B. Wilson in 1905. Wilson developed the technique from earlier reports at the request of Dr William Mayo, surgeon and one of the founders of the Mayo Clinic [3] Earlier reports by Dr Thomas S. Cullen at Johns Hopkins Hospital in Baltimore also involved frozen section, but only after ...
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Louis Blanchard Wilson (December 22, 1866 – October 5, 1943) [1] was an American pathologist and the chief of pathology at Mayo Clinic from 1905 to 1937. Wilson is most famous for initiating the routine use of the frozen section procedure for rapid intraoperative diagnosis.
The thin frozen sections are mounted on a glass slide, fixed immediately & briefly in liquid fixative, and stained using the similar staining techniques as traditional wax embedded sections. The advantages of this method is rapid processing time, less equipment requirement, and less need for ventilation in the laboratory.
The five main characters of the franchise in Olaf's Frozen Adventure.From left to right: Kristoff, Anna, Elsa, Sven, and Olaf. This is a list of characters from Disney's Frozen franchise, which consists of the animated films Frozen (2013) and Frozen 2 (2019), several short films and specials, and other media appearances.
Modern frozen section method. Frozen section histology does not give the added margin of safety by the cytotoxic Mohs paste, [14] originally used by Mohs. This paste might have destroyed any residual cancer cells not detected by the pathologist. Missing epidermal margins. Ideally, the Mohs section should include 100% of the epidermal margin ...
Frozen section procedure: tissue embedded in optimal cutting temperature compound, mounted on a chuck in a cryostat and ready for section production. Optimal cutting temperature compound (OCT compound) is used to embed tissue samples prior to frozen sectioning on a microtome-cryostat. This process is undertaken so as to mount slices (sections ...
Frozen section procedure: water-rich tissues are hardened by freezing and cut in the frozen state with a freezing microtome or microtome-cryostat; sections are stained and examined with a light microscope. This technique is much faster than traditional histology (5 minutes vs 16 hours) and is used in conjunction with medical procedures to ...