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[1] [2] Capillary electrochromatography is a combination of two analytical techniques, high-performance liquid chromatography and capillary electrophoresis. Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube , which is filled with the analyte.
Capillary electrophoresis is a separation technique which uses high electric field to produce electroosmotic flow for separation of ions. Analytes migrate from one end of capillary to other based on their charge, viscosity and size. Higher the electric field, greater is the mobility.
Capillary electrophoresis (CE) is a family of electrokinetic separation methods performed in submillimeter diameter capillaries and in micro- and nanofluidic channels.Very often, CE refers to capillary zone electrophoresis (CZE), but other electrophoretic techniques including capillary gel electrophoresis (CGE), capillary isoelectric focusing (CIEF), capillary isotachophoresis and micellar ...
Microfluidic Sanger sequencing is a lab-on-a-chip application for DNA sequencing, in which the Sanger sequencing steps (thermal cycling, sample purification, and capillary electrophoresis) are integrated on a wafer-scale chip using nanoliter-scale sample volumes. This technology generates long and accurate sequence reads, while obviating many ...
Electrophoresis, separates organic molecules based on their different interaction with a gel under an electric potential (i.e., different travel) Capillary electrophoresis; Electrostatic separation, works on the principle of corona discharge, where two plates are placed close together and high voltage is applied. This high voltage is used to ...
More detailed description and several applications of KCE methods (measuring equilibrium and rate constants of molecular interactions, quantitative affinity analysis of proteins, thermochemistry of protein–ligand interactions, selection of aptamers, determination of temperature inside a capillary) can be found in a PDF presentation: KCE is a conceptual platform for kinetic homogeneous ...
The DNA fragments that result are then separated and detected using electrophoresis. There are two common methods of separation and detection, capillary electrophoresis (CE) and gel electrophoresis. Each STR is polymorphic, but the number of alleles is very small. Typically each STR allele will be shared by around 5 - 20% of individuals.
One example is capillary electrophoresis, [10] [12] in which electric fields are used to separate chemicals according to their electrophoretic mobility by applying an electric field to a narrow capillary, usually made of silica. In electrophoretic separations, the electroosmotic flow affects the elution time of the analytes.